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Complete Schafer, 1974; Pratt omission of M g from the medium gives levels of pelletability in extracts from red-irradiated tissue that 2+ are indistinguishable from those of dark controls. 2 + post-homogenization addition of M g to Moreover, M g - f r e e extracts of red-irradiated Avena as early as 3-4 min after homogenization has been found to be ineffective in permitting the effect of the prior irradiation to be measured (Pratt and Marme, 1976; L. H. Pratt, pers. ). As can be deduced from 2+ the earlier discussion here it is uncertain whether the Mg preserves a pre-existing intracellular association or induces an artifactual one, albeit predetermined in the cell by the irradiation history of the tissue.
Quail, P. H. and E. Schafer (1974) J. Membr. Biol. 15, 393-404. Quail, P. , D. Marme and E. ) 245, 189-191. Schafer, E. (1975) Photochem. Photobiol. 21, 189-191. Schafer, E. (1976) In Light and Plant Development (Edited by H. Smith) pp. 45-59. Butterworth, London. Schafer, E , T. U. Lassig and P. Schopfer (1975) Photochem. Photobiol. 22, 193-202. Yu, R. and J. Carter (1976) J. Exp. Bot. 27, 283-293. , N. Fuad and D. J. Carr (1976) Plant Cell Physiol. 17, 1131-1139. Photochemistry and Photobiology, 1978, Vol.
Moreover, in 2+ either case, it would appear that the stabilization effect of the M g could be either (a) direct in the sense of participating in the inter-molecular bonds 2+ or (b) inlinking the pigment to its binding partner; direct, as would be the case were M g to stabilize a configuration in phytochrome and/or its binding partner conducive to the observed association. Models Various formalistic models have been advanced over the past few years in attempts to rationalize the main features of the enhanced pelletability phenomenon (Quail et al, 1973; Boisard et al, 1974; Schafer, 1976).