By Sterling Chaykin (auth.), Seymour Rothchild (eds.)
Radioactive tracers have had a profound effect on medical study prior to now two decades, really in bio chemistry and allied fields. The swift evolution of refined instrumentation and labeling strategies in the past ten years has made attainable on a regimen foundation exquisitly delicate experiments which with different suggestions will require her culean efforts at the a part of the investigator. The Symposia on Advances in Tracer method, that have been held usually considering that 1957, offer for learn employees in biochemistry, pharmacology, endocrinology, and medication a spotlight at the a variety of advancements within the tracer box which are frequently scattered in the course of the medical literature. quantity three is arecord of the complaints of the 9th and 10th Symposia, that have been subsidized by means of the hot England Nuclear company and the Packard software corporation, Inc. contemporary advances in tracer method, as mirrored by way of the papers during this quantity, were in most cases excited by im proved analytical thoughts, corresponding to liquid scintillation count number ing, autoradiography, and chromatography, and with novel ap plications of categorized compounds in biochemical and medical problems.
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Extra resources for Advances in Tracer Methodology: Volume 3 A collection of papers presented at the Ninth and Tenth Symposia on Tracer Methology
For tracer studies, specific activities of 10 to 50 mc per mmole are sufficient as a rule. For this reason, we generally carry out the reaction with highly active barium carbonate and dilute with benzene, which is used direct1y as solvent. If another solvent is used, it does not necessarily have to be separated afterward, provided that the next reaction can also be performed in the same solvent. Benzene derivatives, too, can be produced in a similar manner by adding certain proportions ofmethylacetylene or ethylacetylene to the radioactive acetylene.
Science 108:558 (1948). 11. ,]. Pharmacol. Exp. Therap. 110:244 (1954). 12. , Second International Symposium on the Chemistry of Natural Products, Prague (August 27 to September 2, 1962). 13. , Monatsber. Deut. Akad. Wiss. Berlin 5:299 (1963). 14. , Chem. Ber. 95:2557 (1962). 15. Straub, W•• Biochem. Z. 28:392 (1910). 16. , International Conference on Medicina1 Plant Research, Sofia (September 16-18, 1963). 17. Rabitzsch, G•• Naturwissenschaften 50:225 (1963). A SIMPLE METHOD OF PREPARING LABELED BENZENE K.
Because of the insolubility of the intermediate complex in both cases there is no reduction of the carboxyl or carbomethoxy group. Finally the successful procedure involves the protection of the double bond by bromine addition (Fig. 3). Acrylic acid in ether solution is quantitatively converted into methyl2,3-dibromopropionate-carboxyl-C 14 by successive additions ofbromine and diazomethane. LiAIH 4 reduction of this ester at -80'C is unsatisfactory, giving rise to a mixture of propanol, allyl alcohol, and 2,3-dibromopropanol.